Plasmid

Part:BBa_I739203:Design

Designed by: Stefan Luzi   Group: iGEM07_ETHZ   (2007-10-21)


pBR322BB2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NotI site found at 3612
    Illegal NotI site found at 3645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3433
    Illegal SapI site found at 2350


Design Notes

Source

Bioprocess Laboratory at ETH Zurich

References